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With exacerbation of brucellosis in the KLA, the following can be detected. An increase in the total number of leukocytes (the norm is up to 9.0 x 10 9 /l). Leukocytes are cells of the body's immune system that respond to the introduction of foreign agents. However, it is worth noting that sometimes with brucellosis, the number of leukocytes remains normal (this is typical for older people with a weakened immune system). Increase in the number of monocytes (normal - 3 - 9%).

Monocytes are a type of leukocytes that pass from the blood to the tissues of various organs, turning into macrophages. It is macrophages that are responsible for the absorption and digestion of ingested brucella, so patients with brucellosis may experience moderate monocytes (an increase in the number of monocytes in the blood) several weeks after infection. Reducing the number of neutrophils. It is a nonspecific but common laboratory sign of brucellosis. An increase in the erythrocyte sedimentation rate (the norm is up to 10 mm per hour in men and up to 15 mm per hour in women).

This laboratory indicator can be used to assess the degree of activity of the inflammatory process in the body. When the immune system is activated, the so-called acute-phase inflammatory proteins are released into the blood, which are attached to the surface of erythrocytes (red blood cells), facilitating their adhesion to each other, as a result of which they quickly settle to the bottom of the test tube during the study.

When foreign microorganisms (bacteria, pathogenic fungi, and any others) enter the body, the immune system reacts in a certain way, producing specific antibodies. These antibodies enter the bloodstream and bind only to the foreign microorganism against which they were synthesized (that is, antibodies against Brucella will not interact with any other bacteria). Based on this, it follows that the more Brucella in the body, the higher the amount of antibodies synthesized against them in the blood serum. This principle is based on many diagnostic methods called serological.

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When serological diagnosis of brucellosis can be used. Wright's reaction (agglutination reaction). Wright's reaction becomes positive already from the first weeks of the disease and can be used for diagnostic purposes throughout the acute period of best casinos.

The essence of this reaction is that when Brucella antigens (special complexes located on the surface of bacteria) interact with specific antibodies, they precipitate. To carry out the reaction, a specially prepared suspension of antigens is placed in a test tube, after which a certain amount of card games online serum is added to be examined. If there are antibodies against Brucella in the test serum (that is, if the patient's body has been in contact with these bacteria and his immune system has begun to fight against them), they will interact with antigens and precipitate, which will be seen when evaluating the results. If there are no antibodies in the patient's blood, no reaction will occur. Coombs reaction. In chronic brucellosis, the formation of so-called incomplete antibodies is possible, which are not detected by the agglutination reaction.

However, these antibodies (which are immunoglobulins) are fixed on many cells of the human body, including red blood cells. The essence of the Coombs reaction is the addition of specific reagents to the test blood that interact with incomplete antigens. If there are any on the surfaces of red blood cells, red blood cells will stick together, that is, the reaction will be positive. Burne test. The essence of this test is as follows. The patient is injected intradermally with a very small dose of brucellosis antigens and the subsequent reaction is observed.

If the patient has never been sick and is not sick with brucellosis, his immune system is not prepared to fight these antigens (it takes at least several weeks to produce specific antibodies). There will be no pronounced reactions (perhaps a short and painless reddening of the skin in the injection area, which is a natural reaction of real casino slots to a foreign substance).

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If the patient is infected with brucellosis, his immune system actively produces antibodies against the antigens of this pathogen.

Intradermal administration of such antigens will cause a pronounced local allergic reaction, which will be manifested by redness, swelling and soreness topain at the injection site for 24-48 hours. This test will be positive already 20 to 30 days after infection (when there may be no obvious clinical signs of the disease).

The essence of this study is to isolate pathogens from various biological tissues of the body (from the blood, from the punctates of the lymph nodes, from the cerebrospinal fluid, and so on). To identify Brucella, the test material is sown on special nutrient media and grown under special conditions for a long time.

  1. Microbiological diagnosis of brucellosis.
  2. It should be noted that due to the complexity of the conduct and frequent false-negative results, as well as due to the high infectiousness of the test material, this study is carried out extremely rarely (it can only be performed in specially equipped laboratories and specially trained personnel).
  3. Differential diagnosis is carried out in order to distinguish brucellosis from diseases that have a similar clinical picture.
Cynthia Flores, LCSW
Acute brucellosis should be differentiated. From malaria. With this pathology, fever is also characterized by a wave-like course, however, there is evidence of the patient's stay in the foci of the spread of this disease (usually tropical countries), as well as the bites of mosquitoes that carry malaria.